TY  - JOUR
A1  - Riedl, Simon A. B.
A1  - Völkl, Matthias
A1  - Holzinger, Anja
A1  - Jasinski, Julia
A1  - Jérôme, Valérie
A1  - Scheibel, Thomas
A1  - Feldhaar, Heike
A1  - Freitag, Ruth
T1  - In vitro cultivation of primary intestinal cells from Eisenia fetida as basis for ecotoxicological studies
Y1  - 2021-11-17
VL  - 31
IS  - 2
SP  - 221
EP  - 233
JF  - Ecotoxicology
DO  - 10.1007/s10646-021-02495-2
PB  - Springer US
N2  - The earthworm Eisenia fetida is a commonly used model organism for unspecific soil feeders in ecotoxicological studies. Its intestinal cells are the first to encounter possible pollutants co-ingested by the earthworm, which makes them prime candidates for studies of toxic effects of environmental pollutants on the cellular as compared to the organismic level. In this context, the aim of this study was to demonstrate the suitability of preparations of primary intestinal E. fetida cells for in vitro ecotoxicological studies. For this purpose, a suitable isolation and cultivation protocol was established. Cells were isolated directly from the intestine, maintaining >85% viability during subsequent cultivations (up to 144 h). Exposure to established pollutants and soil elutriates comprising silver nanoparticles and metal ions (Cu2+, Cd2+) induced a significant decrease in the metabolic activity of the cells. In case of microplastic particles (MP particles), namely 0.2, 0.5, 2.0, and 3.0 µm diameter polystyrene (PS) beads as well as 0.5 and 2.0 µm diameter polylactic acid (PLA) beads, no active uptake was observed. Slight positive as well as negative dose and size dependent effects on the metabolism were seen, which to some extent might correlate with effects on the organismic level.
UR  - http://resolver.sub.uni-goettingen.de/purl?gldocs-11858/11199
ER  -