TY  - JOUR
A1  - Manz, Werner
A1  - Arp, Gernot
A1  - Schumann-Kindel, Gabriela
A1  - Szewzyk, Ulrich
A1  - Reitner, Joachim
T1  - Widefield deconvolution epifluorescence microscopy combined with fluorescence in situ hybridization reveals the spatial arrangement of bacteria in sponge tissue
Y1  - 2000
VL  - 40
IS  - 2
SP  - 125
EP  - 134
JF  - Journal of Microbiological Methods
DO  - 10.1016/S0167-7012(99)00103-7
DO  - 10.23689/fidgeo-2576
N2  - Widefield deconvolution epifluorescence microscopy (WDEM) combined with fluorescence in situ hybridization (FISH) was performed to identify and characterize single bacterial cells within sections of the mediterranean sponge Chondrosia reniformis. Sponges were embedded in paraffin wax or plastic prior to the preparation of thin sections, in situ hybridization and microscopy. Serial digital images generated by widefield epifluorescence microscopy were visualized using an exhaustive photon reassignment deconvolution algorithm and three-dimensional rendering software. Computer processing of series of images taken at different focal planes with the deconvolution technique provided deblurred three-dimensional images with high optical resolution on a submicron scale. Results from the deconvolution enhanced widefield microscopy were compared with conventional epifluorescent microscopical images. By the application of the deconvolution algorithm on digital image data obtained with widefield epifluorescence microscopy after FISH, the occurrence and spatial arrangement of Desulfovibrionaceae closely associated with micropores of Chondrosia reniformis could be visualized.
UR  - http://resolver.sub.uni-goettingen.de/purl?gldocs-11858/6889
ER  -