TY  - JOUR
A1  - Davison, Angus
A1  - McDowell, Gary S.
A1  - Holden, Jennifer M.
A1  - Johnson, Harriet F.
A1  - Koutsovoulos, Georgios D.
A1  - Liu, M. Maureen
A1  - Hulpiau, Paco
A1  - Van Roy, Frans
A1  - Wade, Christopher M.
A1  - Banerjee, Ruby
A1  - Yang, Fengtang
A1  - Chiba, Satoshi
A1  - Davey, John W.
A1  - Jackson, Daniel J.
A1  - Levin, Michael
A1  - Blaxter, Mark L.
T1  - Formin Is Associated with Left-Right Asymmetry in the Pond Snail and the Frog
Y1  - 2016
VL  - 26
IS  - 5
SP  - 654
EP  - 660
JF  - Current Biology
DO  - 10.1016/j.cub.2015.12.071
DO  - 10.23689/fidgeo-2601
N2  - While components of the pathway that establishes left-right asymmetry have been identified in diverse animals, from vertebrates to flies, it is striking that the genes involved in the first symmetry-breaking step remain wholly unknown in the most obviously chiral animals, the gastropod snails. Previously, research on snails was used to show that left-right signaling of Nodal, downstream of symmetry breaking, may be an ancestral feature of the Bilateria [1, 2]. Here, we report that a disabling mutation in one copy of a tandemly duplicated, diaphanousrelated formin is perfectly associated with symmetry breaking in the pond snail. This is supported by the observation that an anti-formin drug treatment converts dextral snail embryos to a sinistral phenocopy, and in frogs, drug inhibition or overexpression by microinjection of formin has a chirality-randomizing effect in early (pre-cilia) embryos. Contrary to expectations based on existingmodels [3–5],wediscovered asymmetric gene expression in 2- and 4-cell snail embryos, preceding morphological asymmetry. As the formin-actin filament has been shown to be part of an asymmetry-breaking switch in vitro [6, 7], together these results are consistent with the view that animals with diverse body plans may derive their asymmetries from the same intracellular chiral elements [8].
UR  - http://resolver.sub.uni-goettingen.de/purl?gldocs-11858/6914
ER  -