TY  - JOUR
A1  - Sleight, Victoria A.
A1  - Marie, Benjamin
A1  - Jackson, Daniel J.
A1  - Dyrynda, Elisabeth A.
A1  - Marie, Arul
A1  - Clark, Melody S.
T1  - An Antarctic molluscan biomineralisation tool-kit
Y1  - 2016
VL  - 6
SP  - 36978
JF  - Scientific Reports
DO  - 10.1038/srep36978
DO  - 10.23689/fidgeo-2605
N2  - The Antarctic clam Laternula elliptica lives almost permanently below 0 °C and therefore is a valuable and tractable model to study the mechanisms of biomineralisation in cold water. The present study employed a multidisciplinary approach using histology, immunohistochemistry, electron microscopy, proteomics and gene expression to investigate this process. Thirty seven proteins were identified via proteomic extraction of the nacreous shell layer, including two not previously found in nacre; a novel T-rich Mucin-like protein and a Zinc-dependent metalloprotease. In situ hybridisation of seven candidate biomineralisation genes revealed discrete spatial expression patterns within the mantle tissue, hinting at modular organisation, which is also observed in the mantle tissues of other molluscs. All seven of these biomineralisation candidates displayed evidence of multifunctionality and strong association with vesicles, which are potentially involved in shell secretion in this species.
UR  - http://resolver.sub.uni-goettingen.de/purl?gldocs-11858/6918
ER  -