TY  - JOUR
A1  - Wrede, Christoph
A1  - Walbaum, Ulrike
A1  - Ducki, Andrea
A1  - Heieren, Iris
A1  - Hoppert, Michael
T1  - Localization of Methyl-Coenzyme M reductase as metabolic marker for diverse methanogenic Archaea.
Y1  - 2013
VL  - 2013
JF  - Archaea (Vancouver, B.C.)
DO  - 10.1155/2013/920241
DO  - 10.23689/fidgeo-2680
N2  - Methyl-Coenzyme M reductase (MCR) as key enzyme for methanogenesis as well as for anaerobic oxidation of methane represents an important metabolic marker for both processes in microbial biofilms. Here, the potential of MCR-specific polyclonal antibodies as metabolic marker in various methanogenic Archaea is shown. For standard growth conditions in laboratory culture, the cytoplasmic localization of the enzyme in Methanothermobacter marburgensis, Methanothermobacter wolfei, Methanococcus maripaludis, Methanosarcina mazei, and in anaerobically methane-oxidizing biofilms is demonstrated. Under growth limiting conditions on nickel-depleted media, at low linear growth of cultures, a fraction of 50-70% of the enzyme was localized close to the cytoplasmic membrane, which implies "facultative" membrane association of the enzyme. This feature may be also useful for assessment of growth-limiting conditions in microbial biofilms.
UR  - http://resolver.sub.uni-goettingen.de/purl?gldocs-11858/6993
ER  -